You need to render whatever works (looks right) with your editing system. You can render either upper field first or lower field first. DV-based editing systems, for instance, are lower field dominant, so if you’re working in Final Cut Pro with miniDV footage, or Avid DV, or Premiere, you should render lower field first.
If you are unsure whether to render upper or lower, try this: create a composition with the NTSC-DV or NTSC-D1 preset, depending on your system (if you don’t know which one you should use, try doing both and this might help you figure that out as well). Create a quick animation of some little circles or text moving quickly across the screen (so it takes, say, two seconds to cross), over and over (a horizontal hail storm), for about 10 seconds. Render this out upper field first (label it upperFirst.mov, or upperFirstDV.mov if you’re doing DV and D1). Render it again lower field first. (You set the field order in the Render settings of your render queue. You don’t need to make any changes to the composition itself.) Import these movies into your editing program, drop them in a timeline, hook it up to a TELEVISION (not a computer monitor) and see which looks better. If you do this right, it should be extremely obvious. It may take a few attempts to get the right codecs and sizes, but that’s all a part of the fun. Good luck,
ben