Brandon,
A little difficult to tell without knowing what all you have going on in your
comp, but a few guesses..
Is your footage interlaced? It appears to be from the still image. You might try
de-interlacing first (compressor does a great job of this if your on a mac)
or here is a tutorial on how to do it in AE https://library.creativecow.net/articles/kramer_andrew/deinterlace.php makes accurate masking a lot easier.
In After effects in the upper right hand of your composition window is a pulldown menu.
Select “enable motion blur” so that you can see if any motion blur is having an effect on your comp while you preview. now when you preview does it still line up?
Andy