If you started at 640 x 480, you should keep top field first all the way through. Make sure After Effects is interpreting your footage as top field first, render it out as top field first, then select interlaced and top field first in Bitvice.
Also, what codec are you rendering to in After Effects? Some codecs have their own field order dialog boxes, so you have to check that,too.
I use Cleaner to stretch M100 640 x 480 movies to 720 x 480 using the motion JPEG B codec, and keep it top field first. Then go to Bitvice. That works great. I don’t use the DVNC or ‘studio rgb’ setting, and it looks fine.
“Hey Pauly, Don’t I need to select “interlaced” if I’m coming from a M100 8.2.2 640X480 orig. It’s scaled in AE to 720 and switched to lower field. I’ve tried 8 and 10 bit quality and settings of 0 or 20 DVNC – none of which help the look or diversely effect compression time. Could the gamma boost and DV Color boost be causing video anomalies and noise around titles that looks like the “Predator” cloaking effect? Thanks, Chris K.”